Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12540/70
Title: An Arabidopsis thaliana leucine-rich repeat protein harbors an adenylyl cyclase catalytic center and affects responses to pathogens
Authors: Bianchet, Chantal 
Wong, Aloysius 
Quaglia, Mara 
Alqurashi, May 
Gehring, Chris 
Ntoukakis, Vardis 
Pasqualini, Stefania 
Issue Date: 2019
Publisher: Elsevier
Source: Bianchet, C., Wong, A., Quaglia, M., Alqurashi, M., Gehring, C., Ntoukakis, V., & Pasqualini, S. (2019). An Arabidopsis thaliana leucine-rich repeat protein harbors an adenylyl cyclase catalytic center and affects responses to pathogens. Journal of plant physiology, 232, 12-22.
Journal: Journal of Plant Physiology
Abstract: Adenylyl cyclases (ACs) catalyze the formation of the second messenger cAMP from ATP. Here we report the characterization of an Arabidopsis thaliana leucine-rich repeat (LRR) protein (At3g14460; AtLRRAC1) as an adenylyl cyclase. Using an AC-specific search motif supported by computational assessments of protein models we identify an AC catalytic center within the N-terminus and demonstrate that AtLRRAC1 can generate cAMP in vitro. Knock-out mutants of AtLRRAC1 have compromised immune responses to the biotrophic fungus Golovinomyces orontii and the hemibiotrophic bacteria Pseudomonas syringae, but not against the necrotrophic fungus Botrytis cinerea. These findings are consistent with a role of cAMP-dependent pathways in the defense against biotrophic and hemibiotrophic plant pathogens.
URI: https://hdl.handle.net/20.500.12540/70
DOI: 10.1016/j.jplph.2018.10.025
Appears in Collections:Scholarly Publications

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